However, a change in attitude is yet to be achieved as the focus of the use of phyto-based products should emphasize, as does TCM, on a holistic approach, thus focusing on the analysis of the entire body functions, performance and health rather than on focusing on disease treatment.
When one tries to read and analyze the multitude of scientific reports written about the topic, the major challenge is to understand which of the phytogenics’ properties - flavoring, antioxidant, antifungal, antiviral, antibacterial, antidepressant, immune modulating and physiological, amongst others, is responsible for their performance-enhancing effect in animals. Moreover, the fact that most of these reports are written with basis on data generated by the use of specific commercially available blends of ingredients rather than on single active substances makes it difficult to ascertain which exact component is responsible for which effect.
Comparisons and conclusions are only possible if trials are performed with the same blend of ingredients, thus, this document summarizes information gathered by different trials performed in poultry which show the effects of BIOMIN’s specific product line of phytogenics – Digestarom® PEP - at different levels.
Gut microflora modulation – Antimicrobial action
The well-known antimicrobial mode of action of botanicals is considered to arise mainly from the potential of the hydrophobic essential oils to intrude into the bacterial cell membrane, to disintegrate membrane structures and cause ion leakage (2). Modulation of microbial activity is of great relevance. It reduces pathogenic microbial pressure in the gastro-intestinal tract thus limiting the competition between the animal and the microbes for nutrients. In turn, production of digestion by-products, such as ammonia, is reduced. Besides its great negative impact on the environment, ammonia is considered the most harmful gas in broiler chicken housing as it irritates respiratory airways and predisposes chickens to respiratory infections, causes keratoconjunctivitis and reduces bacterial clearance from lungs (3). Likewise, endogenous biogenic amines, known to decarboxylate limiting essential amino acids thus rendering them unavailable for growth and performance, are not desirable digestion by-products. Similarly to the results obtained in piglets (4) the use of Digestarom® PEP in poultry reduced total VFA, amines and ammonia were reduced in relation to an antibiotic growth promoter (AGP)-supplemented group (Figure 1). Such results were accompanied by a statistically significant (P<0.05) reduction of the FCR in comparison with both the negative control (-11 points) and the AGP group (-9 points) (data not shown).
Figure 1: Results of a scientific trial conducted at the National Chiayi University in Taiwan. 80 birds/treatment (20 birds/replicate; 4 replications/treatment). 3 treatment groups as follows: Negative Control; Positive Control (OTC 50 ppm and CTC 50 ppm); Digestarom® PEP (125 g/t).
Other tests further confirm the anti-microbial effect of the product against specific bacterial oral challenges. Besides greatly reducing the mortality of birds, intestinal clostridia levels and intestinal lesion scores were significantly lowered by the supplementation of the phytogenic product in Clostridium perfringens challenged broilers (Table 1).
Table 1: Results of 3 trials conducted at USDA, Food and Feed Safety Research Unit, USA. 50 birds/treatment in a total of three treatments, as follows: Negative control; Positive Control (C. perfringens at 1.5 ml of 107 CFU/mL); Trial Group (C. perfringens at 1.5 ml of 107 CFU/mL + Digestarom® PEP (125 g/t)).
Specific impact of phytogenics in gut functions - Stimulation of endogenous enzyme activity
There is evidence that phytogenic feed additives may favorably affect gut functions (optimize passage rate of ingesta, activity of digestive enzymes and nutrient absorption) (2). Preliminary results generated from a research project with an acknowledgeable European research institute show that BIOMIN’s phytogenic product line improved maltase, sucrase and amino peptidase activity by 27 %, 26 % and 27 %, respectively, in comparison with the non-supplemented group (scientific report to be soon released). These enzymes have important roles both in carbohydrate and protein digestion.
Growth promoting efficacy - Enhanced digestibility
Following the ban of antimicrobials as growth promoters in animal diets Kroismayr et al. tested Digestarom® PEP in piglet diets as a potential antibiotic growth promoters (AGP) replacement (4). His findings on improvement in apparent digestibility of organic matter and crude protein were later on confirmed in poultry diets (Figure 2-3). Phytogenics performed better than the mixture of the two synthetic antimicrobials improving final body weight and FCR of birds (data not shown).
Figure 2-3: Results of a trial conducted at UNIOESTE University in Brazil. 384 birds/treatment (24 broilers/replicate; 16 replicates/treatment). 3 treatment groups as follows: AGP (Enramycin 10 ppm + Salinomycin 125 ppm); Digestarom® PEP (125 g/t); Negative Control.
Effects on gut morphology
Changes in gut morphology may be especially important when animals are challenged with very costly diseases such as coccidiosis and/or necrotic enteritis. Typically, infected enterocytes turn over more quickly and therefore are not able to fully differentiate, a fact that severely impairs digestive enzyme secretion thus reducing digestibility of nutrients and rendering animals more susceptible to osmotic and secretory diarrhea (5). BIOMIN’s phytogenic product line increased villus length:crypt depth ratio in broilers challenged with Paracox-5® vaccine (Figure 4). Concomitantly, the number of goblet cells was significantly increased (P<0.05) (data not shown). These factors are proof of the increased absorptive surface and of the improved protection of the gut provided by Digestarom® PEP both reflected on the higher final body weight and on the improved FCR of supplemented animals (6).
Figure 4: Results of a trial conducted at BIOMIN’s R&D station in Austria. 165 birds/treatment (32 broilers/replicate; 8 replicates/treatment). Birds orally challenged with Paracox-5® vaccine. 2 treatment groups, as follows: Challenged group; Challenge + Digestarom® PEP supplementation.
There is a lack of knowledge on the exact mechanism of AGP. Most arguments point toward an anti-inflammatory role of AGP, which reduces energy waste and spares energy for production (7). Based on this, effective alternatives for AGP should share similar properties. The impact of Digestarom® Product Line components on intestinal epithelial cells was investigated by challenging them in vitro with TNFα (Tumor necrosis factor α), a cytokine involved in systemic inflammation. Results showed a down regulation of intercellular adhesion molecule-1 (ICAM-1), interleukin-8 (IL-8), and monocyte chemo-attractant protein-1 (MCP-1), all circulating inflammatory mediators (Figure 5). Conversely, Digestarom® up-regulated anti-inflammatory Cytochrome P450 1A1 (CYP1A1), Heme oxygenase 1 (HO-1), uridine diphosphate glucuronosyltransferase (UGT1A1), all target genes in intestinal cells involved in cell protection (Figure 6).
Figure 5: Results of in vitro scientific studies at Giessen University, Germany. Caco-2 intestinal epithelial cells were challenged with TNFα and the expression of the different pro-inflammatory mediators was studied.
Figure 6: Results of in vitro scientific studies at Giessen University, Germany. The Nrf2-Keap system, one of the major cellular defence mechanisms against oxidative and xenobiotic stresses, was studied.
Further considerations to the use of phytogenics
Further considerations for the use of phytogenics include nutrient sparing effects, increase of total antioxidant capacity of meat and increase juiciness of meat as per sensory quality panel evaluation (data available but not shown). The first is an interesting possibility either for feed mills wanting to manage their costs by down-specifying their diets or for producers who would like to increase the animal’s final weight while maintaining the formulation. The latter ones will reflect on a longer shelf life of meat originating from supplemented birds and on better acceptance of the final product by customers.
Giving a definite answer on the exact mode of action of phytogenics in animals is probably as difficult as replying to the famous, still unanswered question, "which came first, the chicken or the egg?". Nonetheless, one cannot dismiss the variety of data available showing very positive impacts of carefully selected and blended phytogenic components on animal performance and health. As basic needs of the world population are met, consumers will become more focused on the quality of products they ingest. Products providing similar results to antibiotic growth promoters in terms of performance - without their acknowledged negative impacts on bacterial resistance – will be imperative for a sustainable production.
Digestarom® Product Line has proven to achieve and even to exceed AGP results with regard to animal performance. Complementary to that, the combination of plant-derived substances brings extra beneficial parameters which certainly cannot be obtained with the use of synthetic substances. Finally, if you dare to think holistically, make sure you choose a product which is fully backed up with sound technical information and which obeys to proper quality control standards.
To maximize space available, only selected data were presented in this article. Full data regarding individual trials mentioned through the article are available upon request.